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Image Search Results
Journal:
Article Title: Dual requirement for yeast hnRNP Nab2p in mRNA poly(A) tail length control and nuclear export
doi: 10.1093/emboj/21.7.1800
Figure Lengend Snippet: Fig. 5. Targeting of Pab1p to the nucleus suppresses the nab2Δ growth defect. (A) Suppression of nab2Δ by low-copy pPAB1NL. Plate growth of NAB2, nab2Δ pGAL::NAB2, nab2Δ pPAB1NL and nab2Δ pPAB1mnl strains on YPGal (galactose) and YPD (glucose) at 24°C is shown. (B) Immunoblot analysis of NAB2, nab2Δ pPAB1NL and nab2Δ pPAB1mnl strains grown on YPD (glucose) and YPGal (galactose). Proteins were detected using anti-V5 (αV5), anti-Pab1p 1G1 (αPab1p) and anti-Nab2p 3F2 (αNab2p) antibodies. (C) SV40 NLS-tagged Pab1p accumulates in the nucleus. The localizations of V5-tagged Pab1p, poly(A)+ RNA and DNA are compared in NAB2, nab2Δ pPAB1 and nab2Δ pPAB1NL strains.
Article Snippet: Immunoblotting was performed as described ( Anderson et al ., 1993b ) using the following antibodies and dilutions: anti-Nab2p 3F2 (1:500), anti-Pub1p 2B1 (1:5000), anti-Pab1p 1G1 (1:5000),
Techniques: Western Blot